Reprint: Precision Measurement Institute group, Chinese Academy of Sciences Youth Innovation Promotion Association
Recently, precision measuring wangjie (green will promote members) researcher and Xia He spring, dean of ningxia medical university general hospital and led by the scientific research team and the cooperation, to the nerve damage and repair in the field of neuroscience hot and difficult problem, in spinal cord injury and repair process has been studied on the influence of metabolic information in the functional brain regions.
Different from conventional metabolomics and metabolite concentration, cerebral metabolic dynamics is a variable that describes the correlation between neurons and neural activity and signal transmission in the short term, and its principle is shown in Figure 1.Although the total amount of metabolites was the same in A and B, there was A significant difference in the rate of concentration change, that is, B>A, which meant that the neuronal activity of state B was significantly stronger than that of state A, which could not be achieved only by the concentration of metabolites or conventional metabolomics strategies.In this project, we used 13C glucose magnetic resonance probe to label brain neurotransmitters, and use nuclear magnetic resonance spectroscopy [1H-13C]NMR (POCE) to detect the 13C enrichment rate of glucose metabolites at various levels in various brain regions of rat brain in fixed time.Compared with conventional 13C-NMR, POCE technique has higher resolution and faster detection time for 13C labeled substances in samples.
FIG. 1 Correlation diagram of metabolic kinetics and metabolite concentration This study deeply analyzed the influence of spinal cord injury on metabolic information in various functional areas of the brain from the perspective of metabolic kinetics (See FIG. 2).In the experiment, 13C isotopic labeling was used to study the metabolic kinetics of rats, and the metabolite concentration and metabolic kinetics of each brain region were measured based on NMR during acute and chronic spinal cord injury.The results showed that there were weak intergroup differences in the concentration of metabolites in each brain region in acute and chronic periods.However, different metabolites showed significant changes in the metabolic dynamics of multiple brain regions during the acute phase, especially in cortical regions, thalamus, and medulla oblongata.After four weeks of recovery, some of the altered areas of the brain, such as the temporal cortex, occipital cortex and medulla oblata, returned to normal.Metabolism kinetic rate change reveals the rats after spinal cord injury in different brain regions of metabolism and neurotransmitter metabolism, significant changes have taken place in this for spinal cord injury affects the brain activity and glucose metabolism and utilization based on facts, and display the function of spinal cord injury to the brain has a certain degree of influence, for the clinical diagnosis and treatment of spinal cord injury provides a theoretical basis.
Regional BODILY levels and Turnover inawake Rats after Acute or Chronic Spinal Cord Injury (CPA) were published in the Journal FASEB Journal (IF=5.391, AREA 2 of THE Chinese Academy of Sciences).
CIL 13C labeled glucose products are perfect for this experiment